plvx ef1alpha ires mcherry (TaKaRa)
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plvx ef1alpha ires mcherry
Plvx Ef1alpha Ires Mcherry, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+mcherry+c1/pLVX-EF1alpha-AcGFP1-C1+Vector/us12551547-314-10-11
Average 95 stars, based on 14 article reviews
Plvx Ef1alpha Ires Mcherry, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+mcherry+c1/pLVX-EF1alpha-AcGFP1-C1+Vector/us12551547-314-10-11
Average 95 stars, based on 14 article reviews
plvx ef1alpha ires mcherry - by Bioz Stars,
2026-10
95/100 stars
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Amplification:Article Title: In vitro assembly, positioning and contraction of a division ring in minimal cells Article Snippet: .. Then, mCherry gene was amplified from Article Title: The MinDE system is a generic spatial cue for membrane protein distribution in vitro Article Snippet: The coding region for mCherry was amplified by PCR from the plasmid pLVX-mCherry-C1 (Clontech, Mountain View, CA, USA) using the primers 64_BSMTS-mCH_fw/68_His-mcherry_rev and the backbone was amplified from pET28a using the primers pET28a_fw/65_BSMTS-pET_rev. .. The two PCR products overlap and were assembled using seamless assembly. pET28a-mCherry-GGBsMTS encodes for the C-terminal fusion of the B. subtilis MinD MTS (GSGKGMMAKIKSFFGVRS; AA 254-268 of B. subtilis MinD) to mCherry and an N-terminal His-Tag (mCh-MTS(BsD)). mCherry coding region was amplified from Sequencing:Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: Analytes were chromatographically resolved by isocratic elution (90% (B) at 350 μl/min) with an Agilent Eclipse Plus C-8 analytical column (50 × 2.1-mm inner diameter × 1.8-μm particle). .. The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: .. Lentiviral Vector Packaging Using 293FT Cells The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Expressing:Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: Analytes were chromatographically resolved by isocratic elution (90% (B) at 350 μl/min) with an Agilent Eclipse Plus C-8 analytical column (50 × 2.1-mm inner diameter × 1.8-μm particle). .. The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: .. Lentiviral Vector Packaging Using 293FT Cells The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Plasmid Preparation:Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: Analytes were chromatographically resolved by isocratic elution (90% (B) at 350 μl/min) with an Agilent Eclipse Plus C-8 analytical column (50 × 2.1-mm inner diameter × 1.8-μm particle). .. The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Article Title: Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells Article Snippet: .. Lentiviral Vector Packaging Using 293FT Cells The D4 cDNA coding sequence was subcloned into the HIV-based lentiviral expression vector, Polymerase Chain Reaction:Article Title: The MinDE system is a generic spatial cue for membrane protein distribution in vitro Article Snippet: The coding region for mCherry was amplified by PCR from the plasmid pLVX-mCherry-C1 (Clontech, Mountain View, CA, USA) using the primers 64_BSMTS-mCH_fw/68_His-mcherry_rev and the backbone was amplified from pET28a using the primers pET28a_fw/65_BSMTS-pET_rev. .. The two PCR products overlap and were assembled using seamless assembly. pET28a-mCherry-GGBsMTS encodes for the C-terminal fusion of the B. subtilis MinD MTS (GSGKGMMAKIKSFFGVRS; AA 254-268 of B. subtilis MinD) to mCherry and an N-terminal His-Tag (mCh-MTS(BsD)). mCherry coding region was amplified from other:Article Title: Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence Article Snippet: Rabbit anti-MTOR (2983S) was from Cell Signaling Technology. |